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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/16483


    Title: Ca(2+) movement and apoptosis induced by deltamethrin in Madin-Darby canine kidney canine renal tubular cells
    Authors: Fang-Jin Liu;Chiang-Ting Chou;Jin Shiung Cheng;Hong-Tai Chang;Wei-Zhe Liang;Chun-Chi Kuo;Soong-Yu Kuo;Daih-Huang Kuo;Pochuen Shieh;Fang-Rong Chang;Chung-Ren Jan
    Contributors: 輔英科技大學 醫學檢驗生物技術系
    Keywords: Apoptosis;Ca2+;Deltamethrin;Madin–Darby canine kidney;Renal cells
    Date: 2015-01-01
    Issue Date: 2015-08-24 20:32:21 (UTC+8)
    Abstract: This study explored the effect of deltamethrin, a pesticide, on free Ca2+ concentration [Ca2+]i, viability, and apoptosis in Madin–Darby canine kidney (MDCK) canine renal tubular cells. Deltamethrin at concentrations between 10μM and 40μM evoked [Ca2+]i rises in a concentration-dependent manner. The Ca2+ entry was inhibited by nifedipine, econazole, phorbol 12-myristate 13-acetate, and SKF96365. Treatment with the endoplasmic reticulum Ca2+ pump inhibitor 2,5-di-tert-butylhydroquinone (BHQ) in a Ca2+-free medium abolished deltamethrin-induced [Ca2+]i rise. Treatment with deltamethrin also abolished BHQ-induced [Ca2+]i rise. Inhibition of phospholipase C (PLC) activity with U73122 abolished deltamethrin-evoked [Ca2+]i rise. Deltamethrin killed cells at 30–60μM in a concentration-dependent manner. The cytotoxic effect of deltamethrin was not reversed by prechelating cytosolic Ca2+ with the acetoxymethyl ester of 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid. Annexin V/propidium iodide staining data suggest that 30–50μM deltamethrin induced apoptosis. Together, in MDCK renal tubular cells, deltamethrin induced [Ca2+]i rises that involved Ca2+ entry through protein kinase C-mediated store-operated Ca2+ channels, and PLC-dependent Ca2+ release from the endoplasmic reticulum. Deltamethrin also induced Ca2+-independent cell death that might involve apoptosis.
    Relation: Kaohsiung Journal of Medical Sciences 31(1),1-8
    Appears in Collections:[醫學檢驗生物技術系] 期刊論文

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