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    Please use this identifier to cite or link to this item: http://ir.fy.edu.tw/ir/handle/987654321/8295

    Title: Overexpression of circulating c-met messenger RNA is significantly correlated with nodal stage and early recurrence in non-small cell lung cancer.
    Authors: CHENG Tian-Lu;CHANG Mei-Yin;HUANG Sung-Yu;SHEU Chau-Chyun;KAO Eing-Long;CHENG Yu-Jen;CHONG Inn-Wen
    Contributors: 輔英科技大學 醫學檢驗生物技術系
    Keywords: Molecular biology;Malignant tumor;Lung disease;Bronchus disease;Lung cancer;Polymerase chain reaction;Real time;Recurrence;Relapse;Early stage;Messenger RNA;Respiratory disease;Cardiovascular disease;Non small cell carcinoma;non-small cell lung carcinoma
    Date: 2005-09-01
    Issue Date: 2010-10-26 15:02:45 (UTC+8)
    Abstract: Background: The c-met receptor and its ligand hepatocyte growth factor have been shown to be involved in tumor invasiveness and metastasis. Overexpression of c-met has been demonstrated in lung cancer tissues and cell lines, but the expression of c-met in peripheral blood (circulating c-met) has not been addressed. The molecular monitoring of circulating c-met could be helpful for selecting patients for adjuvant therapy. Objectives: To investigate the expression of circulating c-met in non-small cell lung cancer (NSCLC) patients and to assess its prognostic implications. Methods: We quantified the levels of c-met messenger RNA (mRNA) in paired tumor and normal lung tissues and their peripheral bloods in 45 patients with NSCLC by real-time polymerase chain reaction (PCR). The expression status of c-met protein in tumor tissues was further evaluated by immunohistochemistry. Results: c-Met mRNA was significantly higher by 1.5 to 11 times in 34 of 45 tumor tissues (75.5%) than it was in their normal counterparts by real-time PCR. A comparison of this assay to immunohistochemistry suggested that real-time PCR was more sensitive than immunohistochemistry (27 of 45 tumor tissues, 60.0%) for the detection of c-met (p = 0.016). Of these patients with overexpression of c-met in tumors, 67.6% (23 of 34 patients) expressed higher amounts of circulating c-met by 1.4 to 8 times that of the normal control subjects. In addition, overexpression of circulating c-met was significantly correlated with nodal (N) stage (p = 0.011), but weakly correlated with tumor (T) stage (p = 0.056) and overall stages (p = 0.054) in patients with NSCLC. However, no correlations were found among circulating c-met and other factors such as age, gender, and pathologic types. Moreover, by univariate analysis, circulating c-met overexpression and pathologic stages (including T and N stages) were the most important factors correlated with early recurrence (p < 0.05). Only the circulating c-met remained as an independent predictor of early recurrence (hazard ratio, 3.94; 95% confidence interval, 1.17 to 13.33; p = 0.027) after Cox regression multivariate analysis. Conclusions: Overexpression of circulating c-met is significantly correlated with the N stage and early recurrence. Moreover, early recurrence is frequently noted in patients with overexpression of circulating c-met, indicating that circulating c-met is an independent negative prognostic indicator in NSCLC.
    Relation: chest 128(3),1453-1460
    Appears in Collections:[醫學檢驗生物技術系] 期刊論文

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