Please use this identifier to cite or link to this item:
|Title: ||Combined effects of terazosin and genistein on a metastatic hormone-independent human prostate cancer cell line.|
|Authors: ||Chang,Kee-Lung;Cheng, Hsiao-Ling;Huang,Li-Wen;Hsieh,Bau-Shan;Hu,Yu-Chen;Chih,Tsai-Tung;Shyu,Huey-Wen;Su,Shu-Jem|
|Contributors: ||輔英科技大學 健康美容學位學程|
|Keywords: ||Genistein;Terazosin;Prostate cancer;Apoptosis;Vascular endothelial growth factor (VEGF)|
|Issue Date: ||2010-11-11 11:21:37 (UTC+8)|
|Abstract: ||Metastatic prostate cancer progresses from androgen-dependent to androgen-independent. Terazosin, a long-acting selective α1-adrenoreceptor antagonist, induces apoptosis of prostate cancer cells in an α1-adrenoreceptor-independent manner, while genistein, a major soy isoflavone, inhibits the growth of several types of cancer cells. The present study was designed to test the therapeutic potential of a combination of terazosin and genistein using a metastatic, hormone-independent prostatic cancer cell line, DU-145.
Terazosin or genistein treatment inhibited the growth of DU-145 cells in a dose-dependent manner, whereas had no effect on normal prostate epithelial cells. Addition of 1 μg/ml of terazosin, which was inactive alone, augmented the growth inhibitory effect of 5 μg/ml of genistein. Co-treatment with terazosin resulted in the genistein-induced arrest of DU-145 cells in G2/M phase being overridden and an increase in apoptotic cells, as evidenced by procaspase-3 activation and PARP cleavage. The combination also caused a greater decrease in the levels of the apoptosis-regulating protein, Bcl-XL, and of VEGF165 and VEGF121 than genistein alone.
In conclusion, the terazosin/genistein combination was more effective in inhibiting cell growth and VEGF expression as well as inducing apoptosis of the metastatic, androgen-independent prostate cancer cell line, DU-145, than either alone. The doses used in this study are in lower and nontoxic anticancer dosage range, suggesting this combination has potential for therapeutic use.
|Relation: ||Cancer Letters 276(1),14-20|
|Appears in Collections:||[健康美容系] 期刊論文|
Files in This Item:
All items in FYIR are protected by copyright, with all rights reserved.