English  |  正體中文  |  简体中文  |  Items with full text/Total items : 6047/14565 (42%)
Visitors : 13641392      Online Users : 307
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/15460


    Title: Application of real-time quantitative polymerase chain reaction tomonitoring infection of classic swine fever virus and determiningoptimal harvest time in large-scale production
    Authors: Lin,Ya-Ching;Wu, Sheng-Chi;Yang, Ming-Yu;Chen, Guan-Ting;Li, Tzung-Han;Liau, Ming-Yi
    Contributors: 輔英科技大學 生物科技系
    Keywords: CSFV;Vaccine;Non-cytopathogenic;Real-time quantitative PCR assay;Optimum harvest time;Mass production
    Date: 2013-11-01
    Issue Date: 2014-08-08 11:30:02 (UTC+8)
    Abstract: Due to the non-cytopathogenic replication of classical swine fever virus (CSFV) in cell culture, large-scale production of CSFV using bioreactor system remains the problem of monitoring the time of maximum virus production for optimal harvest. In this study, we proposed the application of real-time quantitative PCR assay to monitoring the progress of CSFV infection and yield determination in large scale. The region of NS5B of CSFV responsible for CSFV genome replication was used for the designation of primers and probe. Viral titers determined by the real-time quantitative PCR assay were compared with the conventional cell-culture based method of immunofluorescent staining. Results from large scale production show that a similar profile of CSFV production was successfully outlined by real-time quantitative PCR and virus yields were comparable to the results from immunofluorescent staining assay. By using this method, an optimal harvesting time of the production could be rapidly and precisely determined leading to an improvement in virus harvest.
    Relation: Vaccine , Volume 31, Issue 47, p.5565-5571
    Appears in Collections:[生物科技系] 期刊論文

    Files in This Item:

    File SizeFormat
    index.html0KbHTML585View/Open


    All items in FYIR are protected by copyright, with all rights reserved.


    本網站典藏內容為學術研究目的之提供,請尊重著作權人之權益合理使用,請勿任意重製、轉貼、改作及散佈。

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback