Capillary zone electrophoretic separations of cold medicine ingredients, including acetaminophen (AC), dextromethorphan (DMF) and racemates of phenylpropanolamine (PPA) and chlorpheniramine maleate (CPM) using randomly sulfated-β-CD (S-β-CD) as an electrolyte modifier and a chiral selector were investigated. The results indicate that S-β-CD is an excellent chiral selector and a suitable electrolyte modifier as well for the separation of those cold medicine ingredients. Influences of S-β-CD concentration and buffer concentration on the separation were examined. Baseline separation of these cold medicine ingredients with 1.0?% (w/v) S-β-CD could be simultaneously and successfully achieved within 11.8 minutes. In addition, S-β-CD could also act as a chiral selector for enantioseparation of PPA and CPM. A high enantioselectivity was obtained for these two analytes. Linear relationships between the peak area and its concentration for the calibration curves of AC and DMF were obtained (correlation coefficients: 0.9987 for AC, 0.9965 for DMF, respectively). The relative standard deviations of the migration time and peak area of AC and DMF were 0.19, 2.44?% and 0.34, 2.99?%, respectively. Detection limits were 0.93 and 2.57?μg/mL for AC and DMF, respectively. Recoveries of AC and DMF ranging between 102.42 and 97.28?% were observed. The proposed method was successfully applied to the determination of AC and DMF in cold medicines.
Journal of the Chinese Chemical Society 62(2),191-196