Fooyin University Institutional Repository:Item 987654321/16496
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 6024/14565 (41%)
造访人次 : 14174084      在线人数 : 324
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻
    主页登入上传说明关于FYIR管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.fy.edu.tw:8080/ir/handle/987654321/16496


    题名: Vibrio vulnificus RtxA1 modulated calcium flux contributes reduced internalization in phagocytes
    作者: Soong-Yu Kuo;Miao-Chen Chou;Shin-Luen Lee;Yu Wang;Chun-Liang Chen;Pei-Ting Lin;Horng-Ren Lo
    贡献者: 輔英科技大學 醫學檢驗生物技術系
    关键词: Vibrio vulnificus;RtxA1;Internalization;Cytotoxicity;Calcium flux
    日期: 2015-04-01
    上传时间: 2015-08-24 20:32:42 (UTC+8)
    摘要: Aims
    Vibrio vulnificusis an opportunistic pathogen that causes primary septicemia and wound infection with high mortality rate. This pathogen produces an RTX toxin (RtxA1) which can cause host cell rounding, cell death and interference with internalization by host phagocytes. However, the mechanism of RtxA1-induced phagocyte paralysis is not clear.
    Main methods
    Using the murine macrophage cell line RAW264.7, we measured cytotoxicity and phagocytosis ofV. vulnificusin normal and calcium-depleted media. To deplete extracellular and cytosolic Ca2 +, cells were exposed to the calcium chelators ethylene glycol tetraacetic acid (EGTA) and 1,2-bis-(o-aminophenoxy)-ethane-N,N,N′,N′-tetraacetic acid, tetraacetoxymethyl esteris (BAPTA-AM), respectively. The cytotoxicity was examined by measuring the activity of lactate dehydrogenase (LDH) released from the damaged cells. The gentamicin protection assay was conducted to determine the number of internalized bacteria, while acridine orange staining was applied to visualize the intracellular bacteria. The fluorescent indicator fura-2-acetoxymethyl ester (fura 2-AM) was used to measure the Ca2 +signal post-infection.
    Key findings
    We revealed that extracellular Ca2 +was essential for phagocytes to internalizeV. vulnificus. Meanwhile, cytosolic Ca2 +flux in RAW264.7 cells induced by an RtxA1 isogenic mutant was repressed by the parent strain. Furthermore, depletion of extracellular Ca2 +level by EGTA significantly reduced the cytotoxicity but did not affect the antiphagocytic activity of RtxA1 toxin.
    Significance
    Our results indicated that RtxA1 may interfere with cytosolic Ca2 +flux of phagocyte to promote bacteria colonization.
    關聯: Life Sciences 0(132),55-60
    显示于类别:[醫學檢驗生物技術系] 期刊論文

    文件中的档案:

    没有与此文件相关的档案.



    在FYIR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    本網站典藏內容為學術研究目的之提供,請尊重著作權人之權益合理使用,請勿任意重製、轉貼、改作及散佈。

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈