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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/16668


    Title: Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer
    Authors: Hou, Yu-Yi;You, Jyun-Jie;Yang, Cheng-Mei;Pan, Hung-Wei;Chen, Hung-Chih;Lee, Jang-Hwa;Lin, Yaoh-Shiang;Liou, Huei-Han;Liu, Pei-Feng;Chi, Chao-Chuna;Ger, Luo-Ping;Tsai, Kuo-Wang
    Contributors: 輔英科技大學 生物科技系
    Keywords: DNA methylation;microRNA;migration;oral cancer
    Date: 2016-04-25
    Issue Date: 2016-11-16 15:13:10 (UTC+8)
    Abstract: MicroRNAs (miRs) are a class of small endogenous non-coding RNAs of ~21-24 nucleotides in length. Previous studies have indicated that miR-196b has either an oncogenic or tumor-suppressive function in various types of cancer. However, the biological role of miR-196b in oral squamous cell carcinoma (OSCC) remains unclear. In the present study, the expression levels of miR-196b were examined in oral cancer tissues and corresponding adjacent normal tissues from 69 OSCC patients using stem-loop reverse transcription-quantitative polymerase chain reaction. The results indicated that miR-196b was significantly overexpressed in OSCC tissues compared with the corresponding adjacent normal tissue samples (64 of 69, 92.7%, P<0.001). Analysis of the methylation status of the miR-196b gene indicated more frequent hypomethylation of the CpG islands located upstream of the miR-196b gene in the OSCC tissues than in the adjacent normal tissues (32 of 69, 46.3%), and the methylation status of miR-196b correlated inversely with its expression levels. Furthermore, the unmethylated status of the miR-196b promoter correlated with poor disease-specific survival in OSCC patients (P=0.035). Functional analysis revealed that ectopic miR-196b expression promoted oral cancer cell migration and invasion abilities, and that silencing of miR-196b could abrogate in vitro migration and invasion of oral cancer cells. Collectively, the present findings indicate that the epigenetic regulation of miR-196b expression plays a crucial role in modulating cell migration and invasion during OSCC progression, and thus may serve as a potential prognosis marker or therapeutic target for OSCC.
    Relation: Oncology letters , 11(6): 4013–4021
    Appears in Collections:[生物科技系] 期刊論文

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