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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/16675

    Title: A novel reductive amination method with isotopic formaldehydes for the preparation of internal standard and standards for determining organosulfur compounds in garlic
    Authors: Tsai, De-Cheng;Liu, Meng-Chieh;Lin, Yi-Reng;Huang, Mei-Fang;Liang,Shih-Shin
    Contributors: 輔英科技大學 生物科技系
    Keywords: Organosulfur compounds (OSCs);Reductive amination;S-allylcysteine (SAC);S-allylcysteinine sulfoxide (alliin);S-ethylcysteine (SEC);S-methylcysteine (SMC)
    Date: 2016-04-15
    Issue Date: 2016-11-16 15:36:19 (UTC+8)
    Abstract: Garlic (Allium sativum) is a long-cultivated plant that is widely utilized in cooking and has been employed as a medicine for over 4000 years. In this study, we fabricated standards and internal standards (ISs) for absolute quantification via reductive amination with isotopic formaldehydes. Garlic has four abundant organosulfur compounds (OSCs): S-allylcysteine, S-allylcysteinine sulfoxide, S-methylcysteine, and S-ethylcysteine are abundant in garlic. OSCs with primary amine groups were reacted with isotopic formaldehydes to synthesize ISs and standards. Cooked and uncooked garlic samples were compared, and we utilized tandem mass spectrometry equipped with a selective reaction monitoring technique to absolutely quantify the four organosulfur compounds.
    Relation: Food Chemistry , Volume 197, Part A, Pages 692–698
    Appears in Collections:[生物科技系] 期刊論文

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