English  |  正體中文  |  简体中文  |  Items with full text/Total items : 6047/14565 (42%)
Visitors : 13654607      Online Users : 335
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/16676

    Title: Nasal commensal Staphylococcus epidermidis counteracts influenza virus
    Authors: Chen, Hui-Wen;Liu, Pei-Feng;Liu, Yu-Tsueng;Kuo, Sherwin;Zhang, Xing-Quan;Schooley, Robert T.;Rohde, Holger;Gallo, Richard L.;Huang, Chun-Ming
    Contributors: 輔英科技大學 生物科技系
    Date: 2016-06-16
    Issue Date: 2016-11-16 15:36:22 (UTC+8)
    Abstract: Several microbes, including Staphylococcus epidermidis (S. epidermidis), a Gram-positive bacterium, live inside the human nasal cavity as commensals. The role of these nasal commensals in host innate immunity is largely unknown, although bacterial interference in the nasal microbiome may promote ecological competition between commensal bacteria and pathogenic species. We demonstrate here that S. epidermidis culture supernatants significantly suppressed the infectivity of various influenza viruses. Using high-performance liquid chromatography together with mass spectrometry, we identified a giant extracellular matrix-binding protein (Embp) as the major component involved in the anti-influenza effect of S. epidermidis. This anti-influenza activity was abrogated when Embp was mutated, confirming that Embp is essential for S. epidermidis activity against viral infection. We also showed that both S. epidermidis bacterial particles and Embp can directly bind to influenza virus. Furthermore, the injection of a recombinant Embp fragment containing a fibronectin-binding domain into embryonated eggs increased the survival rate of virus-infected chicken embryos. For an in vivo challenge study, prior Embp intranasal inoculation in chickens suppressed the viral titres and induced the expression of antiviral cytokines in the nasal tissues. These results suggest that S. epidermidis in the nasal cavity may serve as a defence mechanism against influenza virus infection.
    Relation: Scientific Reports , 6 :27870
    Appears in Collections:[生物科技系] 期刊論文

    Files in This Item:

    File SizeFormat

    All items in FYIR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback