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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/17769


    Title: Carbohydrates–chitosan composite carrier for Vero cell culture
    Authors: Lin, Ya-Ching;Chen, Guan-Ting;Wu, Sheng-Chi
    Contributors: 輔英科技大學 生物科技系
    Keywords: Cell carrier;Carbohydrates;Chitosan;Vero cell
    Date: 2016-12-01
    Issue Date: 2017-09-13 14:10:51 (UTC+8)
    Abstract: In this study, carbohydrate–chitosan composite including glucose–chitosan, sucrose–chitosan and starch–chitosan with varied carbohydrate concentrations were prepared as carriers for Vero cell culture. Our results show that among these composites, 30 % starch–chitosan composite (STC) were the best carriers for the growth of Vero cells. The initial number of attached cells on the surface of composite carriers did not have any significant effect on subsequent cell production. A higher glucose level in the growth medium during the exponential phase of cell growth, however, played an important factor for cell production. Vero cells on the STC carriers were able to convert starch inside the composite carriers into glucose and further utilized the glucose for their growth. Moreover, by crosslink with serum the STC carriers supported an even better cell production in the normal medium without adding fetal bovine serum, as well as a good extracellular virus production. The STC composite is therefore a promising alternative carrier for Vero cell culture.
    Relation: Cytotechnology , December 2016, Volume 68, Issue 6, pp 2649–2658
    Appears in Collections:[生物科技系] 期刊論文

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