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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/18828

    Title: A standard addition method to quantify histamine by reductive amination and hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry
    Authors: Shen, Po-Tsun;Lin, Yi-Reng;Chen, Bing-Hung;Huang, Mei-Fang;Cheng, Chieh-Wen;Shiue, Yow-Ling;Liang, Shih-Shin
    Contributors: 輔英科技大學 生物科技系
    Keywords: Standard addition method;histamine;reductive amination;multiple reaction monitoring;hydrophilic interaction liquid chromatography;mass spectrometry
    Date: 2019-04-20
    Issue Date: 2019-08-28 16:30:24 (UTC+8)
    Abstract: Histamine is an organic nitrogenous compound that acts as a neurotransmitter in the uterus, spinal cord, and brain and is involved in local immune responses. In this study, we developed a fast and simple derivatization method based on reductive amination that can be used to quantify histamine by hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry. Histamine isotope analogs were synthesized via reductive amination. Histamine was modified with H2-formaldehyde to form N-dimethylated histamine to act as a standard or with D2-formaldehyde to form N-dimethylated histamine-d4 to act as an internal standard. Using this method, we achieved a limit of detection of 3.6?ng/mL, a limit of quantification of 7.9?ng/mL, and a linear calibration curve with a coefficient of determination (R2) of 0.9987. Furthermore, the intra-day relative standard deviations ranged from 0.9% to 3.7% and the inter-day relative standard deviations ranged from 2.0% to 17.6%. After derivatization, N-dimethylated histamine showed 382.5% signal enhancement compared to unmodified histamine in mass spectrometry detection. To demonstrate the applicability of this method for biological samples, we utilized standard addition method to quantify histamine in fetal bovine serum and achieved a recovery of 86.7%.
    Relation: European Journal of Mass Spectrometry , 2019
    Appears in Collections:[生物科技系] 期刊論文

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