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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/19106


    Title: Reactive oxygen species mediates the chemopreventive effects of syringin in breast cancer cells
    Authors: Lee, Chien-Hsing;Huang, Chiung-Wei;Chang, Po-Chih;Shiau, Jun-Ping;Lin, In-Pin;Lin, Mei-Ying;Lai, Chih-Cheng;Chen, Chung-Yi
    Contributors: 輔英科技大學 保健營養系
    Date: 2019-08-01
    Issue Date: 2020-09-14
    Abstract: Background

    Syringin (Syr), a phenylpropanoid glycoside extracted from Eleutherococcus senticosus, possesses various biological properties, including anticancer activities. However, the cytotoxicity effects of Syr on breast cancer have not yet been elucidated.


    Purpose

    In this study, we evaluated the anticancer potential of Syr on breast carcinoma and the mechanism involved.


    Study design/methods

    Non-tumorigenic (M10), tumorigenic (MCF7) and metastatic (MDA-MB-231) breast cancer cell lines as well as xenograft model were treated with Syr. Proliferation and cell cycle distribution were evaluated using the MTT, the colony formation assay and flow cytometry. The expression levels of cytotoxicity-related proteins were detected by Western blot.


    Results

    Here, we found that colony formation inhibition, cell cycle arrest in the G2/M phase, down-regulation of X-linked inhibitor of apoptosis protein (XIAP), cleaved poly (ADP-ribose) polymerase (PARP) and caspase-3/9 activation were observed in MCF7 and MDA-MB-231 cells treated with Syr. Moreover, pretreatment with a pan-caspase inhibitor (Z-DEVD-FMK) inhibited Syr-induced apoptosis. In addition, treatment with Syr also increased the production of reactive oxygen species (ROS). However, the antioxidant N-acetyl-cysteine (NAC) reversed the ROS levels and rescued the apoptotic changes. Meanwhile, Syr inhibited the growth of breast cancer xenograft models and dramatically decreased tumor volume without any obvious body weight loss in vivo.


    Conclusion

    Our findings suggest that Syr induces oxidative stress to suppress the proliferation of breast cancer and thus might be an effective therapeutic agent to treat breast cancer.
    Relation: Phytomedicine 61(2019),152844
    Appears in Collections:[保健營養系] 期刊論文

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