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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/2128


    Title: Identification of Nontuberculous Mycobacteria Existing in Tap Water by PCR-restriction Fragment Length Polymorphism.
    Authors: Chiao-tang Chang;Ling-yu Wang;Chen-yi Liao;Shiao-ping Huang
    Contributors: 輔英科技大學 醫學檢驗生物技術系
    Date: 2002-06-01
    Issue Date: 2010-09-26 10:48:59 (UTC+8)
    Abstract: This paper presents the finding of the possible cause of the high false-positive rate in acid-fast staining in histological examinations. Using acid-fast staining, culture, and PCR, acid-fast bacilli were detected in 83.7% of 49 hospital tap water samples and nontuberculous mycobacteria (NTM) were detected in 20.4% of the same 49 samples. The 10 NTM isolates were also identified to the species level using PCR-restriction fragment length polymorphism. Our findings indicate that NTM in hospital tap water are the possible cause of false positives in acid-fast staining and of nosocomial infection in immunocompromised patients.
    Relation: Appl. Environ. Microbiol 68(6),3159-3161
    Appears in Collections:[醫學檢驗生物技術系] 期刊論文

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