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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/2217


    Title: Demonstration of a glycoprotein derived from the 24p3 gene in mouse uterine luminal fluid
    Authors: Sin-Tak CHU;Hsein-Lu HUANG;Jin-Mei CHEN;Yee-Hsiung CHEN
    Contributors: 輔英科技大學 保健營養系
    Date: 1996-01-01
    Issue Date: 2010-09-26 14:12:17 (UTC+8)
    Abstract: A glycoprotein in mouse uterine luminal fluid was purified to homogeneity via a series of purification steps involving Sephadex G-100 chromatography, Sephadex G-50 chromatography and HPLC on a reverse-phase C18 column, in that order. Automated Edman degradation was unable to determine the N-terminal residue of the glycoprotein and the partial sequences determined from its trypsin digests were found to be identical with the protein sequence deduced from 24p3 cDNA. The core protein and the total amount of carbohydrate together gave a molecular mass of 25.8 kDa. Results from the characterization of the glycopeptide bond indicated the presence of N-linked carbohydrate but no O-linked carbohydrate in the protein, which has two potential sites for N-linked carbohydrate at Asn81 and Asn85, as deduced from analysis of the primary structure. The core protein was shown to have a molecular mass equal to that of the putative protein deduced from cDNA, suggesting that this protein may contain no signal peptide. Results of Northern-blot analysis for various tissues of adult mice revealed that the 24p3 gene was expressed in lung, spleen, uterus, vagina and epididymis.
    Relation: Biochemiacl Journal (316),545-550
    Appears in Collections:[保健營養系] 期刊論文

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