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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/3245


    Title: On- column preconcentration and separation of DNA On-column preconcentration and separation of DNA fragments using polymer solutions in the presence of electroosmotic flow
    Authors: Hsieh, Ming-Mu;Tseng, Wei-Lung;Chang,Huan-Tsung
    Contributors: 輔英科技大學 應用化學及材料科學系
    Keywords: Capillary electrophoresis;Preconcentration;Polymer solution;Electroosmotic flow;DNA
    Date: 2000-08
    Issue Date: 2010-09-28 16:53:40 (UTC+8)
    Abstract: We demonstrated DNA preconcentration and separation in the presence of electroosmotic flow (EOF) using poly(ethylene oxide) (PEO) solutions. After injecting large volumes of DNA samples into a capillary filled with free tris(hydroxymethyl)amino-methane (Tris)-borate (TB) buffers, PEO solutions entered the capillary by EOF and acted as sieving matrices. In contrast to conventional methods (in the absence of EOF), controlling the EOF was also useful for resolution optimization. We have found that PEO adsorption on the capillary wall was more pronounced when low ionic strength buffers were used. Thus, the EOF decreased with increasing injection length, which led to longer migration times and changes in resolution and stacking efficiency. All resolution values were higher than 1.5 when 1.0 μg/mL DNA samples were injected at 240 V/cm for 60 s (0.67 μL). In addition, as low as 0.015 μg/mL DNA samples (an about 66-fold increase in sensitivity) were detected when the injection was performed at 250 V/cm for 60 s.
    Relation: ELECTROPHORESIS,Volume 21, Issue 14, pages 2904–2910
    Appears in Collections:[應用化學及材料科學系] 期刊論文

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