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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/3248


    Title: Optimizing separation conditions for polycyclic aromatic hydrocarbons in micellar electrokinetic chromatography
    Authors: Hsieh, Ming-Mu;Kuo,Yui-Chun;Tsai ,Pei-Ling;Chang,Huan-Tsung
    Contributors: 輔英科技大學 應用化學及材料科學系
    Keywords: Polynuclear aromatic hydrocarbons
    Date: 2001-07-21
    Issue Date: 2010-09-28 16:53:40 (UTC+8)
    Abstract: We report the separation of polycyclic aromatic hydrocarbons (PAHs) using 0.1% poly(ethylene oxide) (PEO) in micellar electrokinetic chromatography (MEKC). In the presence of PEO, adsorption of PAHs on the capillary wall was reduced, leading to better resolution and reproducibility. Effects of tetrapentylammonium iodide (TPAI), dextran sulfate (DS), methanol, and sodium lauryl sulfate (SDS) on the separation of PAHs were elucidated. In terms of resolution and speed, DS, compared to TPAI, is a better additive for separation of PAHs. When using 0.1% PEO solution containing 45% methanol, 50 mM SDS, and 0.02% DS, separation of 10 PAHs containing 2 to 5 benzene rings was accomplished in less than 12 min at 15 kV in a commercial CE system. The method has also been tested for separating seven PAHs with high quantum yields when excited at 325 nm using a He–Cd laser. Unfortunately, separation of the seven PAHs was not achieved and sensitivity diminished under the same conditions. To optimize sensitivity, resolution and speed, a stepwise technique in MEKC has been proposed. The seven PAHs were resolved in 35 min at 15 kV when separation was performed in 0.1% PEO solution containing 35 mM SDS, 40% methanol and 0.02% DS for 2 min, and subsequently in 0.1% PEO solution containing 20 mM SDS, 50% methanol, and 0.02% DS.
    Relation: Journal of Chromatography A ,Volume 924, Issues 1-2, Pages 397-405
    Appears in Collections:[應用化學及材料科學系] 期刊論文

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