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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/3267


    Title: Selective enrichment of aminothiols using polysorbate 20-capped gold nanoparticles followed by capillary electrophoresis with laser-induced fluorescence
    Authors: Shen, Chien-Chih;Tseng, Wei-Lung Tseng,;Hsieh,Ming-Mu
    Contributors: 輔英科技大學 應用化學及材料科學系
    Keywords: Capillary electrophoresis;Gold nanoparticles;Enrichment;Aminothiol;o-phthalaldehyde
    Date: 2009-02-01
    Issue Date: 2010-09-28 16:53:43 (UTC+8)
    Abstract: In this article, we report a simple method for selective enrichment of aminothiols using Tween 20-capped gold nanoparticles (AuNPs) prior to capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF). Compared to citrate-capped AuNPs, Tween 20-capped AuNPs exhibit the ability to disperse in a highly saline solution and selectively extract aminothiols through the formation of Au-S bonds. After extraction and centrifugation, 1mM thioglycollic acid (TGA) was utilized to remove aminothiols that attached to the NP surfaces. After a solution of 8.0 mL aminothiols were extracted using 2x AuNPs (200 microL), the extracted aminothiols derivatized with o-phthalaldehyde at pH 12.0 were detected by CE-LIF. As a result, the limits of detection at a signal-to-noise ratio of 3 for homocysteine (HCys), glutathione (GSH), and gamma-glutamycysteine (Glu-cys) are 4013.2, 79.8, and 382.8 pM, respectively. The use of this probe provided approximately 11-, 282-, and 21-fold sensitivity improvements for HCys, GSH, and Glu-cys, respectively. A practical analysis of HCys, GSH, and Glu-cys in human urine sample has been accomplished by this present method.
    Relation: Journal of Chromatography A ,Volume 1216, Issue 2, Pages 288-293
    Appears in Collections:[應用化學及材料科學系] 期刊論文

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