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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/6598


    Title: Radiation inactivation analysis of H+-pyrophosphatase from submitochondrial particles of etiolated mung bean seedlings.
    Authors: Shih Sheng Jiang;Su Jing Yang;Soong Yu Kuo;Rong Long Pan
    Contributors: 輔英科技大學 醫學檢驗生物技術系
    Keywords: Functional size;Radiation inactivation;H+-pyrophosphatase;Proton translocation;Submitochondrial particle
    Date: 2000-03-01
    Issue Date: 2010-10-13 16:11:27 (UTC+8)
    Abstract: Radiation inactivation analysis was employed to determine the functional masses of enzymatic activity and proton translocation of H+-pyrophosphatase from submitochondrial particles of etiolated mung bean seedlings. The activities of H+-pyrophosphatase decayed as a simple exponential function with respect to radiation dosage. D37 values of 6.9�0.3 and 7.5�0.5 Mrad were obtained for pyrophosphate hydrolysis and its associated proton translocation, yielding molecular masses of 170�7 and 156�11 kDa, respectively. In the presence of valinomycin and 50 mM KCl, the functional size of H+-pyrophosphatase of tonoplast was decreased, while that of submitochondrial particles remained the same, indicating that they are two distinct types of proton pump using PPi as their energy source.
    Relation: FEBS Lett. 468(2-3),211-214
    Appears in Collections:[醫學檢驗生物技術系] 期刊論文

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