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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/6623


    Title: Recovery of Salmonella by using selenite brilliant green sulfa enrichment broth.
    Authors: Chiao-tang Chang;Chung-Yee You;Hui-Ching Shen;A-Mai Li;Chao-yu Chen;Jui-ling Chou;Shiao-ping Huang
    Contributors: 輔英科技大學 醫學檢驗生物技術系
    Date: 1999-12-01
    Issue Date: 2010-10-14 09:36:26 (UTC+8)
    Abstract: The efficacy and sensitivity of selenite brilliant green sulfa enrichment (SBG) broth for the isolation of
    Salmonella from fecal specimens were evaluated by using both clinical and artificially infected (artificial) fecal
    specimens. An examination of 1,588 clinical fecal specimens found Salmonella in 296 specimens, including 89
    cases detected by the direct-plating xylose-lysine-desoxycholate method and an additional 207 cases detected
    after enrichment with SBG broth. Therefore, the recovery of Salmonella with SBG broth is increased 3.3-fold
    over that by the direct-plating method alone. Furthermore, the isolation rate of Salmonella is higher when using
    SBG broth than when using gram-negative (GN) broth or GN broth supplemented with sodium selenite. To
    determine the sensitivity for the recovery of Salmonella, artificial specimens containing various amounts of
    Salmonella were prepared and analyzed. The results indicated that the sensitivity is also higher with SBG broth
    than with GN broth. Moreover, the optimal incubation period for SBG broth can be extended to 24 h. In
    conclusion, the SBG enrichment method provides a higher recovery rate of Salmonella from fecal specimens.
    Relation: J. Clin. Microbiol., 37(12),4120-4123
    Appears in Collections:[醫學檢驗生物技術系] 期刊論文

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