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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/7051


    Title: Detection of the Cross-reactivity for Atopic IgE Against Multiple Allergens.
    Authors: Yee-Hsuan Chiou;Chung-Yee You;Lin-Yu Wang;Shiao-ping Huang
    Contributors: 輔英科技大學 醫學檢驗生物技術系
    Date: 2003-03-01
    Issue Date: 2010-10-15 13:54:20 (UTC+8)
    Abstract: The existence of specific immunoglobulin E (IgE) allows us to determine the allergens that cause the allergic disease. For the purposes of allergen avoidance and immunotherapy, the measurement of specific IgE is widely applied in clinical laboratories. However, if IgE from the serum of an allergic patient exhibits reactivity to multiple allergens, it would cause a problem. The present study analyzes whether the serum IgE with multiple reactivity is due to the presence of unique IgE against the common epitope shared by different allergens or the presence of multiple IgEs against different epitopes on different allergens. The quantitative-competitive inhibition tests and the immunoblotting were applied to analyze the immunosimilarity among examined allergens. The result shows that the competitive inhibition of IgE binding between shrimp and crab allergens is higher than those between either shrimp and cockroach or between crab and cockroach. Furthermore, the results of immunoblotting are consistent with those of quantitative-competitive inhibition tests. These results allow us to detect the cross-reactivity for atopic IgE against multiple allergens.
    Relation: Clin. Diag. Lab. Immunol., 10(2),229-232
    Appears in Collections:[醫學檢驗生物技術系] 期刊論文

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