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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/7123


    Title: Identification of induced acidification in iron-enriched cultures of Bacillus subtilis during biosur
    Authors: Wei,Yu-Hong;Wang,Li-Fen;Changy,Jo-Shu;Kung, Shieh-Shiuh
    Contributors: 輔英科技大學 應用化學及材料科學系
    Keywords: surfactin;biosurfactant;iron;acidification;phthalic anhydride
    Date: 2003-09-01
    Issue Date: 2010-10-15 16:35:39 (UTC+8)
    Abstract: Bacillus subtilis is able to synthesize a lipopeptide biosurfactant (known as surfactin), which is one of the most effective biosurfactants available. In our previous study, B. subtilis ATCC 21332 was used to produce surfactant and it was found that the addition of iron at an appropriate amount significantly improved the biosurfactant production. However, it also showed that excess addition of iron led to a sharp decrease in pH of the culture and surfactin in the broth disappeared rapidly once the pH fell below 5.0. This study reveals that the disappearance of surfactin at the acidic pH was due to the precipitation of surfactin triggered by the accumulation of extracellular acidic metabolites. The acidic material was isolated and purified from the broth and was characterized by GC-MS and IR spectroscopy. The results of GC-MS and IR analysis show that the molecular weight and molecular structure of the acidic metabolite resembled those of phthalic anhydride.
    Relation: Journal of Bioscience and Bioengineering,Volume 96, Issue 2, Pages 174-178
    Appears in Collections:[應用化學及材料科學系] 期刊論文

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