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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/799

    Title: Calcium requirement in nitrogen fixation in the cyanobacterium Synechococcus RF-1
    Authors: Chen,Tsung-Hsien;Huang,Tan-Chi;Chow, Te-Jin
    Contributors: 輔英科技大學 生物科技系
    Keywords: Calcium and N2 fixation;Cyanobacteria;Nitrogen fixation and Ca2+;Rhythm, circadian (in N2 fixation);Synechococcus
    Date: 1988-06-01
    Issue Date: 2010-09-24 09:46:17 (UTC+8)
    Abstract: Under diurnal 16/8-h light-dark cycles, ethyleneglycol-bis-(-aminoethyl ether)-N,N,N,N-tetraacetic acid (EGTA) at 1 mM completely blocked the appearance of rhythmic N2-fixing activity in Synechococcus RF-1. Ca2+ at 2 mM, when supplied either together with or several hours after the EGTA application, restored the nitrogenase activity, whereas, when Ca2+ was supplied several hours later, the peak of nitrogenase activity was shifted from the dark to the light period in which the activity is normally suppressed. Sr2+ also reversed the inhibition by EGTA, but only partially. When O2 in the gas phase above the culture was below 1%, the inhibition of nitrogenase activity by EGTA was reduced to less than 20% of the control value without EGTA. Thus Ca2+ appears to be required by the cell to protect its nitrogenase from inactivation by O2. In media without EGTA, a close correlation between nitrogenase activity and concentrations of Ca2+ was also observed.
    Relation: Planta, Volume 173, Number 2, Pages 253-256
    Appears in Collections:[生物科技系] 期刊論文

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