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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/8842


    Title: The garlic ingredient diallyl sulfide induces Ca2+ mobilization in Madin-Darby canine kidney cells.
    Authors: Ching-Hsein Chen;Shu-Jem Su;Kee-Lung Chang;Mei-Wen Huang;Soong-Yu Kuo
    Contributors: 輔英科技大學 醫學檢驗生物技術系
    Keywords: Ca2+ signaling;Diallyl sulfide (DAS);Fura-2;Garlic;MDCK
    Date: 2009-06-01
    Issue Date: 2010-11-05
    Abstract: Diallyl sulfide (DAS), one of the major organosulfur compounds (OSCs) of garlic, is recognized as a group of potential chemoproventive compounds. In this study, we examines the early signaling effects of DAS on renal cells loaded with Ca2+-sensitive dye fura-2. It was found that DAS caused an immediate and sustained rise of [Ca2+]i in a concentration-dependent manner (EC50 = 2.32 mM). DAS also induced a [Ca2+]i elevation when extracellular Ca2+ was removed, but the magnitude was reduced by 45%. Depletion of intracellular Ca2+ stores with CCCP, a mitochondrial uncoupler, did not affect DAS’s effect. In Ca2+-free medium, the DAS-induced [Ca2+]i rise was abolished by depleting stored Ca2+ with thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor). DAS-caused [Ca2+]i rise in Ca2+-containing medium was not affected by modulation of protein kinase C activity. The DAS-induced Ca2+ influx was blocked by nicardipine. U73122, an inhibitor of phospholipase C, abolished ATP (but not DAS)-induced [Ca2+]i rise. Additionally, pretreatment with DAS for 24 h decreased cell viability in a concentration-dependent manner. Furthermore, DAS-induced cell death involved apoptotic events. These findings suggest that diallyl sulfide induced a significant rise in [Ca2+]i in MDCK renal tubular cells by stimulating both extracellular Ca2+ influx and thapsigargin-sensitive intracellular Ca2+ release via as yet unidentified mechanisms.
    Relation: Food and Chemical Toxicology 47(9),2344-2350
    Appears in Collections:[醫學檢驗生物技術系] 期刊論文

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