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    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/9019


    Title: Issues pertaining to the analysis of buprenorphine and its metabolites by gas chromatography–mass spectrometry.
    Authors: Yu-Shan Wang;Dong-Liang Lin;Shu-Ching Yang;Meng-Yan Wu;Ray H. Liu;Lien-Wen Su;Pai-Sheng Cheng;Chiareiy Liu;Ming-Ren Fuh
    Contributors: 輔英科技大學 醫學檢驗生物技術系
    Keywords: Buprenorphine;Norbuprenorphine;Glucuronide;Immunoassay;Gas chromatography–mass spectrometry;Liquid chromatography–tandem mass spectrometry
    Date: 2010-03-01
    Issue Date: 2010-11-05 15:58:26 (UTC+8)
    Abstract: “Substitution therapy” and the use of buprenorphine (B) as an agent for treating heroin addiction continue to gain acceptance and have recently been implemented in Taiwan. Mature and widely utilized gas chromatography–mass spectrometry (GC–MS) technology can complement the low cost and highly sensitive immunoassay (IA) approach to facilitate the implementation of analytical tasks supporting compliance monitoring and pharmacokinetic/pharmacogenetic studies. Issues critical to GC–MS analysis of B and norbuprenorphine (NB) (free and as glucuronides), including extraction, hydrolysis, derivatization, and quantitation approaches were studied, followed by comparing the resulting data against those derived from IA and two types of liquid chromatography–tandem mass spectrometry (LC–MS/MS) methods. Commercial solid-phase extraction devices, highly effective for recovering all metabolites, may not be suitable for the analysis of free B and NB; acetyl-derivatization products exhibit the most favorable chromatographic, ion intensity, and cross-contribution characteristics for GC–MS analysis. Evaluation of IA, GC–MS, and LC–MS/MS data obtained in three laboratories has proven the 2-aliquot GC–MS protocol effective for the determination of free B and NB and their glucuronides.
    Relation: J Chromatogr A 1217(10),1688-1694
    Appears in Collections:[醫學檢驗生物技術系] 期刊論文

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