English  |  正體中文  |  简体中文  |  Items with full text/Total items : 6647/14525 (46%)
Visitors : 12539233      Online Users : 370
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/9630

    Title: Actinodaphnine induces apoptosis through increased nitric oxide, reactive oxygen species and down-regulation of NF-κB signaling in human hepatoma Mahlavu cells.
    Authors: Tian-Jye Hsieh;Tsan-Zon Liu;Fung-Jou Lu;Pei-Ying Hsieh;Ching-Hsein Chen
    Contributors: 輔英科技大學 健康美容學位學程
    Keywords: Actinodaphnine;Hepatoma;Apoptosis;Nitric oxide;Reactive oxygen species;NF-κB
    Date: 2006-03-01
    Issue Date: 2010-11-11 11:21:44 (UTC+8)
    Abstract: Actinodaphnine, extracted from Cinnamomum insularimontanum (Lauraceae), possesses cytotoxicity in some cancers, but the mechanism by which actinodaphnine induces apoptosis in human hepatoma cells remains poorly understood. In this study, we investigated the mechanisms of apoptosis induced by actinodaphnine in human hepatoma Mahlavu cells. Treatment with actinodaphnine dose-dependently induced apoptosis in Mahlavu cells that correlated with increased intracellular nitric oxide (NO) and reactive oxygen species (ROS), disruptive mitochondrial transmembrane potential (ΔΨm), and activation of caspase 3/7. Our data also demonstrated that actinodaphnine down-regulated activity of nuclear factor κB (NF-κB). The apoptotic response to actinodaphnine was markedly decreased in Mahlavu cells pretreated with dexsamethasone, a NO inhibitor, N-acetylcysteine (NAC), an antioxidant, and Boc-Asp(OMe)-fmk, a broad caspases inhibitor. These results suggested that actinodaphnine-induced apoptosis is initially mediated through the NO and/or ROS increase and caspases-dependent pathway. In conclusion, our results indicate that an increase of ROS and/or NO is the initial essential event that results in the decrease of ΔΨm and the activation of caspases that commits the cells to the apoptotic pathway in actinodaphnine-treated hepatoma Mahlavu cells.
    Relation: Food and Chemical Toxicology 44(3),344-354
    Appears in Collections:[健康美容系] 期刊論文

    Files in This Item:

    File Description SizeFormat

    All items in FYIR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback