English  |  正體中文  |  简体中文  |  Items with full text/Total items : 6647/14525 (46%)
Visitors : 12465188      Online Users : 376
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://ir.fy.edu.tw:8080/ir/handle/987654321/9652


    Title: Anticancer activity evaluation of the Solanum glycoalkaloid solamargine: Triggering apoptosis in human hepatoma cells.
    Authors: Kou-Wha Kuo;Shu-Hui Hsu;Yun-Ping Li;Wei-Ling Lin;Li-Feng Liu;Li-Ching Chang;Chih-Chao Lin;Chun-Nan Lin;Hamm-Ming Sheu
    Contributors: 輔英科技大學 健康美容學位學程
    Keywords: solamargine;hepatoma;apoptosis;cell cycle;TNF receptors;drug discovery
    Date: 2000-12-01
    Issue Date: 2010-11-11 14:29:45 (UTC+8)
    Abstract: Solamargine, an herbal and molluscicidal medicine derived from Solanum incanum, is a steroidal alkaloid glycoside. To characterize the anticancer mechanism of solamargine on human hepatoma cells (Hep3B), changes of cell morphology, DNA content, and gene expression of cells after solamargine treatment were studied. The appearance in solamargine-treated cells of chromatin condensation, DNA fragmentation, and a sub-G1 peak in a DNA histogram suggests that solamargine induces cell death by apoptosis. The maximum number of dead Hep3B cells was detected within 2 hr of incubation with constant concentrations of solamargine, and no further cell death was observed after an extended incubation with solamargine, indicating that the action of solamargine was irreversible. To determine the susceptibility of cell phases to solamargine-mediated apoptosis, Hep3B cells were synchronized at defined cell cycles by cyclosporin A, colchicine, and genistein, followed by solamargine treatment. The 50 values of solamargine for control, G0/G1-, M-, and G2/M-synchronized Hep3B cells were 5.0, > 10, 3.7, and 3.1 μg/mL, implying that cells in the G2/M phases are relatively susceptible to solamargine-mediated apoptosis. In addition, a parallel up-regulation of tumor necrosis factor receptor (TNFR)-I and -II on Hep3B cells was detected after solamargine treatment, and the solamargine-mediated cytotoxicity could be neutralized with either TNFR-I or -II specific antibody. Therefore, these results reveal that the actions of TNFR-I and -II on Hep3B cells may be independent, and both are involved in the mechanism of solamargine-mediated apoptosis.
    Relation: Biochemical Pharmacology 60(12),1865-1873
    Appears in Collections:[健康美容系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML775View/Open


    All items in FYIR are protected by copyright, with all rights reserved.


    本網站典藏內容為學術研究目的之提供,請尊重著作權人之權益合理使用,請勿任意重製、轉貼、改作及散佈。

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback